Peasy basic seamless
WebpEASY® -Uni Seamless Cloning and Assembly Kit pEASY ®-Basic Seamless Cloning and Assembly Kit Cloning Competent Cells Trans 10 Chemically Competent Cell Trans 5α Chemically Competent Cell Trans 109 Chemically Competent Cell Trans 110 Chemically Competent Cell Trans 1-Blue Chemically Competent Cell Trans 2-Blue Chemically … WebJun 23, 2024 · Then, the amplification products were digested with XbaI and BamHI and were subcloned into the pAN583:mCherry vector with pEASY ®-Basic Seamless Cloning and Assembly Kit (TransGen Biotech, China), generating the TaMC1-mCherry fusion construct pAN583:TaMC1-mCherry.
Peasy basic seamless
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WebSep 23, 2024 · Then EcoR I linearized vector pColdTF and the five fragments mentioned above were infused by using pEASY®-Basic Seamless Cloning and Assembly Kit to … WebDec 23, 2024 · The coding sequence of the SWEETs and SnRK2s were amplified and cloned into the plant expression vectors pHBT95 (digested with BamHI and PstI) using pEASY-Basic Seamless Cloning and Assembly Kit ...
WebJan 12, 2024 · Expression vectors were constructed using pEASY ®-Basic Seamless Cloning and Assembly Kit (TransGen Biotech, Beijing, China). The whole coding sequences were amplified from cDNA templates of young fruit of ‘Xindajiubao’ using the KOD-plus-neo high-fidelity enzyme kit (Toyobo, Osaka, Japan). WebOct 17, 2024 · The above five fragments were infused by pEASY®-Basic Seamless Cloning and Assembly Kit (Takara, Dalian, Chain). The transformants were screened on LB agar plate with 50 μg/mL ampicillin. Vector pColdTF-rox1-NtCCD10 was extracted and verified by Sma I and Pst I digestion.
WebJan 25, 2024 · Excised stigmas were inserted into basic PGM ... The fragment was cloned into the modified pCAMBIA1300 vector with a HA or GFP tag using pEASY-Basic Seamless Cloning and Assembly Kit (TransGen ... WebThe four amplified fragments were gel purified and assembled into pBac[3×P3-EGFP afm] digested with QuickCut TM BglII using pEASY ®-Basic SeamLess Cloning and Assembly Kit; the 4000-bp genomic fragment was used as the promoter of AsOBP1 gene, and AsOBP1 and EGFP were fused through the T2A linker. The OE vector was classified as pBac[AsOBP1 ...
WebJul 30, 2024 · The open reading frame of genes ccpA and sigA were recombined into pET-28a plasmid via Nco I-Kpn I sites using the pEASY®-Basic Seamless Cloning and …
WebList 10 jobs that AI will create for humans. 1 AI Ethicist - With the increasing role of AI in society, there will be a need for individuals who can evaluate and ensure that AI is used ethically. 2 AI Trainer - AI needs data to learn, and AI trainers will be responsible for selecting and preparing data to train AI algorithms. t9 thermostatsWebMar 8, 2024 · The amplified PCR products were assembled and linked to the shuttle vector pPIC9γ using pEASY-Basic Seamless Cloning and Assembly Kit (TransGen Biotech, Beijing, China), linearized using Bgl II and transformed into P. pastoris GS115. The Af AP signal peptide was predicted using SignalP 4.1 ( http://www.cbs.dtu.dk/services/SignalP/ ). t9 township\\u0027sWebTransGen Biotech pEASY®-Basic Seamless Cloning and Assembly Kit takes advantage of proprietary recombinases and homologous recombination to achieve directional recombination of PCR inserts with 15-25bp overlapping ends into any linearized vector, enabling efficient and seamless assembly of 1-5 fragments. Free delivery in Singapore. t9 thicket\u0027sWebMar 10, 2024 · The coding sequence of PbWRKY31 was inserted into the pBI121-GFP vector under the 35S promoter using pEASY-Basic Seamless Cloning and Assembly Kit (TransGene, Beijing, China). The resulting 35S:PbWRKY31-GFP vector was confirmed via sequencing and transformed into Agrobacterium tumefaciens strain EHA105 (weidibio, … t9 town\\u0027sWebOct 17, 2024 · The above five fragments were infused by pEASY®-Basic Seamless Cloning and Assembly Kit (Takara, Dalian, Chain). The transformants were screened on LB agar … t9 town\u0027sWebJul 30, 2024 · The open reading frame of genes ccpA and sigA were recombined into pET-28a plasmid via Nco I-Kpn I sites using the pEASY®-Basic Seamless Cloning and Assembly Kit (TransGen Biotech, Beijing, China) to construct plasmids pET … t9 township\u0027sWebNov 4, 2024 · We used pEASY®-Basic Seamless Cloning and Assembly Kit (TRANS) to construct the plasmids of pET28a-cisY, pET28a-pncB, pET28a-gpmA, pET28a-mdh, pET28a-pykF, and pBR322-pykF-His tag. For mutation ... t9 velocity\u0027s