WebAug 1, 1999 · The microsomal limonene-6-hydroxylase was purified from the oil glands of spearmint, and amino acid sequences from the homogeneous enzyme were used to … WebJul 1, 1999 · Each binding or import reaction (adapted from Bruce et al., 1994) contained the following: 10 6 dpm of either [35 S]limonene synthase or [35 S]SSU as a control; either 0.1 m m ATP for binding or 4 m m ATP for translocation (final concentration); and intact chloroplasts corresponding to 25 μg of chlorophyll in a final volume of 150 μL. Binding ...
Organization of monoterpene biosynthesis in Mentha ... - PubMed
Weblimonene-6-hydroxylase playing a pivotal role in controlling the nature of the end product. In the youngest stages, when limonene-6-hydroxylase is undetectable, only limonene was accumulating in WebFourteen unigenes related to monoterpenoid biosynthesis were identified, among which (+)-pulegone reductase (PR) and (−)-menthol dehydrogenase (MD) were significantly upregulated in l-menthol chemotype and (−)-limonene 6-hydroxylase was significantly upregulated in carvone chemotype. In addition, 2,599 TFs from 66 families were identified ... microsoft teams paging on video gallery
Identification of Fungal Limonene-3-Hydroxylase for
WebDec 10, 2004 · The ER localization of limonene-6-hydroxylase is consistent with the ER targeting, membrane insertion sequence of this enzyme, and with its demonstrated … WebThe limonene-6-hydroxylase from Mentha spicata (CYP71D18) was used as a query in a blastp search of an EST database generated from peltate trichomes of Origanum vulgare . Completion of matching EST sequences by rapid amplification of complementary DNA (cDNA) ends–PCR in two oregano accessions led to the identification of a gene … WebMar 29, 2024 · There have been only a few reports of amino acid residues controlling regiospecificity: peppermint limonene 3-hydroxylase (GenBank Accession No. AF124817, CYP71D15) and spearmint limonene 6-hydroxylase (GenBank Accession No. AF124815, CYP71D18) show 70% identity and catalyze the regiospecific hydroxylation of limonene … microsoft teams page templates