Designing in vivo active dnazymes

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August undefined, 2024

WebMar 30, 2024 · Designing in vivo active DNAzymes Yifei Zhou, Chuanzheng Zhou Published: 30 March 2024 by Springer Science and Business Media LLC in Nature Chemistry Nature Chemistrypp 1-2; doi:10.1038/s41557-021-00669-3 Publisher Website Full-Text Google Scholar Show/hide abstract The publisher has not yet granted … WebOct 2, 2024 · DNAzymes were previously identified by in vitro selection for a variety of chemical reactions, including several biologically relevant peptide modifications. However, finding DNAzymes for peptide lysine acylation is a substantial challenge. ... By the design of Fig. 2C, a successful DNAzyme-catalyzed acylation reaction joins the acyl donor ...

Designing in vivo active DNAzymes. (2024) Yifei Zhou

WebDec 15, 2013 · G-quadruplex DNAzymes are a promising tool in the design of biosensors and chemical sensors because of their advantages over natural enzymes. The G-quadruplex-forming G-rich sequence, the solution components, the reaction substrate and the G-quadruplex enrichment technique can affect the detection sensitivity. In the future, … WebMar 30, 2024 · Full text; References Similar Articles Designing in vivo active DNAzymes. cocoon 固定ページ投稿者非表示

Designing in vivo active DNAzymes Semantic Scholar

WebDNAzymes, also known as deoxyribozymes or DNA enzymes, refer to single-stranded DNA molecules with catalytic capabilities. DNAzymes are generated de novo by in vitro … WebWe describe available prediction tools that should help in the design of efficient DNAzymes and show some experimental methods to test the predictions. The main example is for a 10–23 DNAzyme, but the procedure works as well for the 8–17 DNAzyme family. less Based on techniques References cocoon 固定ページ投稿日非表示

Designing in vivo active DNAzymes - NASA/ADS

Fabrication of bifunctional G-quadruplex-hemin DNAzymes for ...

WebDec 29, 2024 · The aim is to target unwanted RNA molecules of viruses, cancer, or damaged nerve cells, using DNAzymes to attack and destroy them. This is achieved via binding sequences that match a sequence of nucleotides on the targeted RNA molecule. The DNAzyme docks precisely to the matching position and the core cleaves the RNA … WebFurthermore, by designing an apurinic/apyrimidinic site or a complementary sequence of microRNA-21 (miRNA-21) in long loops, bifunctional G-DNAzymes can be split in the presence of apurinic/apyrimidinic endonuclease 1 (APE1) or miRNA-21, decreasing their peroxidase activities. cocoon 固定ページ背景画像WebMar 25, 2024 · DNA enzymes (DNAzymes), which cleave target RNA with high specificity, have been widely investigated as potential oligonucleotide-based therapeutics. cocoon 固定ページ更新日非表示

"WebJun 10, 2024 · Till then, proteins were the only catalysts known in biology. Later, in the 90’s, the first synthetic genetic polymers made of DNA, DNAzymes, were obtained. Since then, numerous DNAzymes have been designed but its mode of action at atomic resolution and whether they were able to adopt complex and compact geometries remained unclear. " - Designing in vivo active dnazymes

Designing in vivo active dnazymes

Factors influencing the performance of G-quadruplex

WebZhou, Y., & Zhou, C. (2024). Designing in vivo active DNAzymes. Nature Chemistry, 13 (4), 299–301. doi:10.1038/s41557-021-00669-3 10.1038/s41557-021-00669-3 … WebDesigning in vivo active DNAzymes. Designing in vivo active DNAzymes. Designing in vivo active DNAzymes Nat Chem. 2024 Mar 30. doi: 10.1038/s41557-021-00669-3. …

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WebDec 22, 2024 · DNAzymes -- a word made up of DNA and enzyme -- are catalytically active DNA sequences. They comprise a catalytic core comprising around 15 nucleic acids … WebMar 29, 2024 · The therapeutic applications of DNAzymes are limited because of their low effectiveness in vivo. Now, a promising approach for constructing DNAzymes that show …

WebOct 19, 2024 · 1 Introduction. With the discovery of ribozymes in the early 1980s, the search for catalytic DNA or DNAzymes started about a decade later. The first DNAzyme was reported in 1994 with RNA-cleavage activity. 1 To date, a diverse range of DNAzymes have been isolated to catalyze various chemical and biological transformations from DNA/RNA … WebMar 30, 2024 · Now, a promising approach for constructing DNAzymes that show high gene-silencing efficiency in mammalian cells has been developed. This approach incorporates chemical modifications into an ...

WebJun 24, 2024 · Researchers at Illinois have developed a technique that, for the first time, allows DNAzymes to target and cut double-stranded DNA, overcoming a significant limitation of the technology. DNAzymes have been used in biosensing, DNA computing and many other applications. WebThe role of IGF-I signaling in tumor growth has been demonstrated in vivo using nucleic acid-based strategies. Here, we designed the first 10–23 DNAzymes directed against …

WebChemically modified DNAzymes were synthesised by incorporating a modified nucleotide either side of the binding arms. Two types of modifications were used: 3'-3'-inverted thymidine (iT) obtained from Invitrogen and locked nucleic acids (LNA) obtained from Proligo (http://www.proligo.com).

WebMar 30, 2024 · The therapeutic applications of DNAzymes are limited because of their low effectiveness in vivo. Now, a promising approach for constructing DNAzymes that... cocoon 固定ページ日付非表示WebMar 30, 2024 · Here, we designed the first 10-23 DNAzymes directed against IGF-I mRNA. Unlike antisense approaches and RNA interference that require protein catalysis, … cocoon 投稿日付表示されないWebSep 4, 2024 · The development of the “in vitro selection” technique in the early 1990s has led to the isolation of many DNAzymes—man-made single-stranded DNA molecules with catalytic abilities—from synthetic random-sequence DNA pools. This chapter serves to introduce this class of synthetic enzymes. cocoon 広告貼り方サイドバーWebTheranostic DNAzymes Wenhu Zhou1,2, ... DNAzyme can be directed to the cleavage site by designing the substrate binding arms using Watson -Crick base pairing, thus inhibiting gene expression. The general mechanism of the RNA cleavage reaction is also presented, where the 2′-OH group attacks the scissile phosphate to initiate the cleavage ... cocoon 文字サイズ変わらないWebMar 30, 2024 · A promising approach for constructing DNAzymes that show high gene-silencing efficiency in mammalian cells has been developed that incorporates chemical … cocoon 投稿カテゴリー非表示WebZhou, Y., & Zhou, C. (2024). Designing in vivo active DNAzymes. Nature Chemistry, 13(4), 299–301. doi:10.1038/s41557-021-00669-3 cocoon 目次カスタマイズ cssWebMar 20, 2024 · DNAzymes have been used to suppress pathogenic genes in a growing number of disease models (reviewed in Khachigian, 2002; Achenbach et al, 2004) and in other in vivo applications (Zhang et al, 2004; Liu et al, 2005). Understanding the design of efficient DNAzymes is of growing interest in the gene therapy field. cocoon 新着記事カテゴリーラベルカスタマイズ