Cyp19a1b-gfp
WebAug 15, 2013 · Tg(cyp19a1b-GFP) zebrafish embryos as an alternative to in vivo (adult) fish tests for oestrogenicity screening. Zebrafish embryos have detectable expression and … Webcyp19a1b ID ZDB-GENE-001103-4 Name cytochrome P450, family 19, subfamily A, polypeptide 1b Symbol cyp19a1b Nomenclature History Previous Names. CYP19a2 ; …
Cyp19a1b-gfp
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WebThe CYP19A1 gene provides instructions for making an enzyme called aromatase. This enzyme converts a class of hormones called androgens, which are involved in male … WebMar 18, 2024 · Title: A cyp19a1b-gfp (aromatase B) transgenic zebrafish line that expresses GFP in radial glial cells. Cyp19b is regulated by estrogens.Promoter …
WebNov 20, 2024 · The transgene can be integrated into the host genome by several methods, which are the most common: Transposons, mediated by nucleases such as Zinc Fingers nucleases (ZFNs), Transcription activator-like effector nucleases (TALENs), and RNA-guided nucleases such as endonuclease CRISPR/Cas9 (clustered regularly interspersed … WebA cyp19a1b-GFP (aromatase B) transgenic zebrafish line that expresses GFP in radial glial cells Sok Keng Tong, Karen Mouriec, Ming Wei Kuo, Elisabeth Pellegrini, Marie Madeleine Gueguen, François Brion, Olivier Kah, Bon Chu Chung Faculty of Medical Sciences Research output: Contribution to journal› Article› peer-review 103Citations (Scopus)
WebMay 7, 2012 · The tg(cyp19a1b-GFP) transgenic zebrafish expresses GFP (green fluorescent protein) under the control of the cyp19a1b gene, encoding brain aromatase. … Web100transgenic cyp19a1b-GFP zebrafish embryos. Because the skull of early developmental 101stages of zebrafish is transparent, GFP is observed, imaged and quantified in vivo. The intensity of fluorescence is then quantified using image analysis software. 102 10318.
WebWith the development of new technologies, new ways of testing chemicals have emerged and will increasingly develop. These new methods generally include Intellectual Property Rights (IPR). IPR aim to stimulate innovation by enabling inventors to appropriate the returns on their investments.
Webcyp19a1b-GFPzebrafish line, we de- termined thatcypa1bup-regulation by E2 is detectable as early as 25 hpf. Altogether these data indicate that thecyp19a1bgene is a target for es- trogens and xenoestrogens at very early stages of development. RESULTS Early Expression of esr1, esr2b, and esr2a mRNAs earl tracklistWebTg(cyp19a1b:cyp19a1b-GFP) ID ZDB-TGCONSTRCT-110126-4 Name Tg(cyp19a1b:cyp19a1b-GFP) Previous Names None Type engineered_plasmid … earl t pottorffWebMar 11, 2024 · Newly fertilized cyp19a1b-GFP eleutheroembryos were transferred to glass crystallizers and exposed from 0- to 4-dpf in an incubator (28 ± 1°C) under semi-static conditions with a total renewal of the medium each day. Each condition consisted in a triplicate of crystallizers containing 7 embryos and 15 ml of water. css scrollbar on divWebAlthough high GEN concentrations are necessary to induce cyp19a1b expression in the cyp19a1b-GFP transgenic zebrafish assay, response to GEN seems to be more sensitive compared to another in vivo short-term (48 h) zebrafish embryo assay using morphological defects as endpoints (edema, head and tail malformation, reduced spontaneous … css scrollbar right marginWebFeb 1, 2009 · A cyp19a1b‐gfp (aromatase B) transgenic zebrafish line that expresses GFP in radial glial cells. Aromatase is an enzyme that catalyzes the synthesis of estrogen in … earl trading penrithWebSep 15, 2016 · The cyp19a1b -GFP transgenic zebrafish ( Tong et al., 2009) were held at the Institut National de l'Environnement Industriel et des Risques (INERIS, Verneuil-en-Halatte, France). They were maintained in 3.5 L aquaria in a recirculation system (Zebtec, Tecniplast, Buguggiate, Italy) on a 14 h light: 10 h dark cycle at a temperature of 25.1 ± … earl trackerWebDec 19, 2008 · GFP was also coexpressed with radial cell marker BLBP, but was not in neurons. In addition, GFP expression in the radial glial cells was stimulated by estrogen, … css scrollbar on hover